Standard Operating Procedure for Petri Dish

By Laura May 13th, 2026 28 views

1. Prepare and Sterilize
Before use, glass petri dishes are sterilized by high-pressure steam or dry heat sterilization. Disposable plastic petri dishes are pre-sterilized and ready for direct use.

2. Aseptic Preparation
Perform all operations in a laminar flow cabinet. Disinfect the workbench and keep the environment sterile to avoid microbial contamination.

3. Pour Culture Medium
Melt the agar medium and cool it to about 45–50℃. Gently lift the petri dish lid only partially, pour the medium into the dish, then place it horizontally until the agar solidifies completely.

4. Inoculation
Use streak plate method, spread plate method or pour plate method to inoculate bacterial or fungal samples onto the solid medium. Keep the dish lid opened as little as possible during operation.

5. Seal and Label
Cover the petri dish tightly, mark the dish with sample name, date, strain number and culture conditions clearly on the outer bottom.

6. Incubation
Place the petri dish upside down in a constant temperature incubator. Inversion prevents condensed water from dropping to destroy colonies and reduces airborne contamination.

7. Observation and Recording
After incubation, take out the petri dish to observe colony morphology, size, color and transparency, and record experimental results.

8. Post-experiment Disposal
Contaminated petri dishes are autoclaved first to kill microorganisms, then classified as biological waste for safe disposal. Reusable glass dishes are cleaned and re-sterilized for next use.

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Standard Operating Procedure for Petri Dish